Abstract Non insulin dependent diabetes also known as type 2 diabetes mellitus (T2DM) is a major risk factor for cardiovascular disease and can have devastating consequences on quality of life. Diagnosis of Acute cardiac event in the early stage of its onset is important in the treatment process, and the development of highly sensitive and specific immunoassays for myocardial proteins such as cardiac Troponin I (cTnI) and Creatine kinase-MB had made it possible. The identification of subjects with high risk of developing cardiac event in the future is more significant as it will provide time to prevent such incidents. Alcohol is also a risk factor for both diabetes and cardiovascular disorder. This paper is aimed at evaluating the relationship between alcohol and cardiac markers like CK-MB, Troponin I and lipid profile in Non insulin dependent diabetes mellitus subjects. A total of 200 adult male (aged 30-70 years) subjects were selected by stratified random sampling technique and by administration of questionnaire. Glucose, lipid profile, and CK-MB levels of these subjects were assayed using 2012 Shenzhen mindray semi-auto Chemistry Analyzer, model BA-88A. Troponin I was assayed by enzyme linked immunosorbent assay (ELISA) method. The blood glucose levels of both men who reported to be diabetic and non diabetic were assayed to reconfirm those who were diabetics and the non diabetics. Statistical package for Social Sciences (SPSS) version 20.0 software was used to analyze the data obtained in this study. Values were expressed as mean ± standard deviation Level of significant was taken at p<0.05. The results show that Creatine Kinase-MB is significantly higher in diabetics than in non diabetic male subjects (p = 0.004). There are also significantly increased levels of total cholesterol, low density lipoprotein cholesterol, high density lipoprotein cholesterol, diastolic blood pressure, and troponin I in alcoholic type 2 diabetic men when compared to the corresponding non alcoholic diabetics. Also, the result shows that alcohol positively associated with troponin I, triglyceride, and fasting blood sugar in alcoholic diabetics. From this study, lipid profile levels can still be used in screening populations to identify the subjects with high risk of developing cardiac event which is identified by highly sensitive and specific positive Troponin test. Alcoholic diabetic subjects are at increased risk of cardiovascular disease than non alcoholic diabetic subjects with same anthropometric factors.