Abstract Blepharis is a genus in the family Acanthaceae. This genus comprises around 129 species distributed in arid and semi-arid regions. Many species belonging to this genus are used in folklore medicine. Blepharis maderaspatensis is a procumbent or scrambling perennial herb. It is used in folklore medicine against wounds, edema, snake bite and gout. The plant possesses anti-inflammatory and anti-nociceptive properties. An isoflavone has been isolated by thin layer chromatography from the whole plant of Blepharis maderaspatensis. The partial structure of the isolated flavonoid has been deduced on the basis of its spectral data (UV and 1HNMR). Different fractions of Blepharis maderaspatensis have been screened for antimicrobial activity. The ethanolic extract of Blepharis maderaspatensis showed moderate against Bacillus subtilis. Other fractions showed partial activity against Bacillus subtilis. The n-butanol fraction exhibited partial activity against Staphylococcus aureus and Escherichia coli.
Keywords Isolation, Isoflavone, Sudanese Blepharis maderaspatensis (L.), Antimicrobial Activity.