Abstract Phytotherapy is still playing a vital role in primary health care in developing countries and intensive literature reports on the impact of secondary metabolites on human physiology potentiated the applications of medicinal plants. Haplophyllum tuberculatum is a plant of many medicinal attributes.The plantis used traditionally against a wide range of diseases. In this study, the oil from Haplophyllum tuberculatum seeds was analyzed by GC-MS. The GC-MS analysis showed 22 components. Major constituents are: i) 9,12-octadecadienoic acid methyl ester (49.60%); 9,12,15-octadecatrienoic acid methyl ester (17.91%); hexadecanoic acid methyl ester (11.70%) and methyl stereate (8.07%). oil was assessed for antimicrobial activity. It exhibited good antibacterial activity against Bacillus subtilis.
Keywords Haplophyllum tuberculatum, Flavonids, Oil, GC-MS Analysis, Antimicrobial Activity.