Abstract Worldwide, traditional medicine is still playing a vital role in primary health care and numerous pharmacological reports on the impact of bioactive phytochemicals on human physiology even potentiated the applications of medicinal plants.In this study, Acacia nilotica Subsp. nilotica oil has been analyzed by GC-MS. The analysis showed 26 components. Major constituents are : methyl-10-trans-12-cis-octadecadienoate(39.39%); 9-octadecenoic acid methyl ester(20.37%); hexadecanoic acid methyl ester(14.62%) and methyl stearate(11.25%). The antmicrobial activity of the oil has been assessed. At a concentration of 100mg/ml the oil showed significant activity against Bacillus subtilis, Staphylococcus aureus and Pseudomonas aeruginosa. However , it showed no activity against Escherichia coli, Candida albicans and Aspergillus niger.
Keywords Acacia nilotica Subsp. nilotica , Oil , Constituents , Antimirobial Activity.