Abstract A new simple, precise, accurate and cost effective UV spectrophotometric method has been developed for the estimation of Cefpodoxime and Ofloxacin in combined dosage form by simultaneous equation method. This method utilized methanol: 0.1 N HCl (50:50) as common solvent. The wavelength selected for simultaneous equation method for Cefpodoxime shows maximum absorption at 276 nm and Ofloxacin shows maximum absorption at 306.4 nm. The drugs follow the Beer-Lamberts law in the concentration range of 10-30 mcg/ml. The method was validated by following analytical parameters as suggested by the ICH guideline which included accuracy, precision assay, LOD and LOQ. All validation parameters were within acceptable range. The correlation coefficient of Cefpodoxime and Ofloxacin was found to be 0.999. A % recovery was found to be 99.66 % for Cefpodoxime and 99.6 % for Ofloxacin. The proposed method is recommended for routine analysis of cefpodoxime and ofloxacin in its combined dosage forms.
Keywords Cefpodoxime proxetil, Ofloxacin, Simultaneous equation method, Methanol, HCl.