Abstract The Production of cephalosporin C by Acremonium chrysogenumin flask contain fermentation medium, after that its extraction, purification and estimation was performed by Spectrophotometric Method. The antimicrobial activities of both the crude and purified cephalosporin C were performed against the test organisms by Agar Well Diffusion Method. Agar well diffusion method and the inhibition zones were recorded and evaluated the effectiveness of the antibiotic against gram positive bacteria (Staphylococcus aereus and Bacillus subtilis) and no effectiveness against gram negative bacteria (Pseudomonas aeruginosa and Proteus mirabilis).
Keywords Cephalosporin C, Acremonium chrysogenum, Antimicrobial, Well diffusion method.