Abstract Within the framework of diligent and continuous research for alternative tools and botanical products to control Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae). Salvia officinalis (L.) (Lamiales: Lamiaceae) essential oil was assayed as an ovicidal and larvicidal. Chemical analysis of S. officinalis oil using GC-MS analysis showed monoterpenoids 1.8-Cineole (61.80%) and Camphor (17.18%) followed by monoterpenes Camphene (5.64%), α-Pinene (5.18%) and 2-β-Pinene (2.86%). Generally, the results of toxicity tests indicated that 1st instar larvae are more susceptible than 3rd instar one, LC50 and LC90 values were (1.963, 5.993 ppm) and (4.555, 26.073 ppm), respectively. On the other hand, treating three days old eggs possessed LC50 and LC90 21.152, 396.892 ppm respectively. Biological results revealed that the oil elongated the larval duration, reduced pupal weight and increased pupal mortality than control. Meanwhile, the tested oil caused a reduction in AchE and increased LDH enzymes as compared to control.
Keywords Spodoptera littoralis, Salvia officinalis, ovicide, larvicide, enzymes.