Investigation of Reverse Phase HPLC Method for Determination of Nimodipinein Pharmaceutical Dosage Form using Nanoparticles Modified with Cetyltrimethylammonium Bromide

Abstract A rapid and sensitive high-performance liquid chromatogaphic method was developed for the estimation of nimodipine in pharmaceutical dosage forms. Nimodipine was chromatographed on a reverse phase C- 18 column in a mobile phase consisting of acetonitrile and water in the ratio of 58:42 (v/v). The mobile phase was pumped at a flow rate of 1 ml/min and the eluents were monitored at 241 nm. A pre-concentration of nimodipine was applied using Nanoparticles Modified with Cetyltrimethylammonium Bromide. The calibration curve was linear in the range of 0.1-40 µg/mL.  The intra and inter-day variation was found to be less than 1% showing high precision of the assay method. The mean recovery of the from the solution containing 10 µg/mL was 99.95 ± 0.86% indicating high accuracy of the proposed HPLC method. Due to its simplicity, rapidness, high precision and the proposed HPLC method may be used for determining nimodipine in bulk drug samples and pharmaceutical dosage forms.

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Updated: May 8, 2018 — 9:02 am