Abstract Current study aimed to maximize multiplication rate, growth parameters and producing superior plantlets which can tolerate acclimatization conditions of Conadria fig cultivar through utilizing of Thidiazuron (TDZ), isopentenyladenine (2iP) separately. Shoots- that passed establishment stage- were cultured on MS medium that contained different concentrations of TDZ and 2iP (0.5,1 and 1.5 mg/l). In addition, activated charcoal and auxin type indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) were applied in rooting stage to evaluate their influences on root formation. Data indicated that number of shoots was produced on lower concentration of 0.5 mg/l 2iP while, 0.5 mg/l TDZ improved shoot thickness. Also, supplementation of the culture medium with 1.5 mg/l TDZ raised chlorophyll content. However, the optimal combination for number of roots and root length was 1.0 mg/l IBA+0.1mg/l IAA. Meanwhile, using of 1.0 mg/l IBA+0.1 mg/l IAA or 0.1 mg/l IAA increased root percentage (100%) as compared with auxin free medium plus activated charcoal, On another hand no beneficial effect was obtained from adding 0.1 mg/l IAA and1.0mg/l IBA+0.1mg/l IAA to the culture medium when root percentage was concerned. Conclusion of current study was gathering both of 2iP and TDZ since each hormone had a positive impact on proliferation rate, plantlet growth performance and chlorophyll content of fig (Conadria cv.), thereby gathering both of them may lead to promising results. Also, more studies should be carried out to state the optimum combination of TDZ and 2iP to produce ideal proliferation rate with vigor plantlets.
Keywords In vitro proliferation, Conadria fig cultivar, Thidiazuron (TDZ), isopentenyladenine (2iP).