Abstract Egypt is considered the top producer of Jasmine concrete; made from Jasminum officinale L,. The study of the genetic variabilities between different Jasminum species cultivated in Egypt in order to propagate the desired species. In this study, start codon targeted (SCoT) and inter-simple sequence repeats (ISSR) markers were used as genetic markers for the selected Jasminum species. SCoT primers produced out of 28 bands were visualized across the species, the results obtained 28 total bands with molecular weight from 230 to 1815 bp, 6 of total amplified bands were polymorphic (21.42 %) and the highest polymorphic percentage was 44.44 % produced with primer SCoT 1 and the lowest percentage was 16.66 % produced with primer SCoT 2 and SCoT4. ISSR primers obtained 31 total bands with molecular weight from 115 to 3400 bp, 18 of total amplified bands were polymorphic(58.05 %) and the highest polymorphic percentage was 71.42 % produced with primer HB-15 and the lowest was 50 % produced with primer 44B. Unweighted pair group method with arithmetic mean (UPGMA) was used for genetic relationships illustration.All the ISSR and SCoT primers used in the present study allowed for enough distinction among Jasminum species and cultivars.
Keywords DNA; inter-simple sequence repeats (ISSR); start codon targeted (SCoT); Jasminum; Unweighted; pair group method with arithmetic mean (UPGMA).