Abstract To identify some compounds by GC-MS from the biologically active compounds have been identified in hexane-acetone, acetone-methanol and ethyl acetate organic solvents. The determination of bioactive chemical compounds is based on peak area, retention time, molecular weight and molecular formula. From the endophytic bacteria of Houttuynia cordata Thunb from Bacillus subtilis strain B237 having antibacterial activity against Staphylococcus aureus from people’s furuncles. Houttuynia cordata Thunb was collected in Kien Giang province, bacteria were isolated in PDA medium. The investigation of the antibacterial ability of endophytic bacteria of Houttuynia cordata Thunb with Staphylococcus aureus is carried out by diffusion method by filter paper ring and identified by 16S rRNA gene sequencing method of bacteria strains, then compare the sequence of bacterial strains with sequences of bacterial strains in NCBI data bank. Ten bioactive compounds were identified in the organic solvent ethyl-acetate by GC-MS analysis of Bacillus subtilis strain B237 has compounds such as Octadecanoic acid, Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl), Docosanoic acid, ethyl ester, 2,5-Cyclohexadien-1-one, 3,5-dihydroxy-4,4-dimethyl-, 4(1H)-Pyrimidinone, 6-amino-2-methyl-5-nitroso-, Benzenemethanol, 3-hydroxy-5-methoxy-, Hexadecanoic acid, ethyl ester, Pentadecanoic acid, ethyl ester, Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(phenylmethyl)-, Diisooctyl phthalate. Especially, that have antibacterial activity against Staphylococcus aureus, that was Docosanoic acid- ethyl ester; 4(1H)-Pyrimidinone, 6-amino-2-methyl-5-nitroso-; Hexadecanoic acid-ethyl ester; Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(phenylmethyl).
Keywords Antibacterial, bioactive compounds, chemistry of natural compounds, endophytic bacteria, Houttuyniacor data Thunb.