Abstract Objective: To observe antibacterial, antifungal, MIC of two compounds from methanol root extract.
Methodology: The powdered root extracted n-Hexane, acetone, chloroform and methanol using separating funnel. The antibacterial activity of S. cumini were tested against thirteen bacteria following by disc diffusion assay method and Ciprofloxacin was used as a control. Antifungal activity were tested against five pathogenic fungi and Nystatin used as control. Serial dilution technique was used for Minimum Inhibitory Concentrations (MIC).
Results: Ciprofloxacin responsive with highest inhibition of zones at 30 µg/disc concentration. Both the compounds were highly responsive of tested bacteria. Nystatin showed the larger zone of inhibition for all the tested fungi. C. albicans possessed higher responsive zone of inhibition than A. niger but there was no zone of inhibition recorded on other tested fungi. The MIC of the purified compound S2 were 128 µg/ml against S. -β-haemolyticus, S. dysenteriae and B. cereus.
Conclusion: The results summarized that antibacterial and antifungal activities of both the compounds S. cumini against life threatening pathogens which appears to be an effective material for development of antimicrobial drugs and ecofriendly biopesticides.
Keywords Antimicrobial potency, disk diffusion method, compounds, Syzygium cumini.