Abstract Poroplis, a sticky material collected by bees and used in their hives as a general purpose sealer, is well known for its diverse and useful biological activities: antimicrobial, antioxidant, anti-inflammatory, immuno-stimulating, and many others. The aim of the present research was focused on investigating the preliminary phytochemical screening, antibacterial and antifungal activity of Propolisvia in vitro approach. The ethanolic extract of Propolis was tested against E. coli, Shegella sonnei, Pseudomonas aeruginosa and Klebsiella pneumonia by agar well diffusion method and broth dilution method. The antifungal activity was tested with ten different fungal strain A. oryza, A. niger, A. altera, A. flavus, P. chrogin, P. notatum, B. fubae, P. digitatum, F. solanai and F. moliniforme. Results showed promising antibacterial and antifungal activity against the bacterial and fungal strain tested. The ethanolic extract was found to have a more potent inhibitory effect comparing with the standard antibiotics which prove the potentiality of the plant extracts for the treatment of various skin and gastrointestinal infections in humans.
Keywords Propolis, Phytochemical, Antibacterial, Antifungal activity and Pathogens.