Abstract The oil from Raphanus sativus L. seeds was characterized by GC-MS. A total of 25 components were identified and quantified. Major constituents are: 13-docosenoic acid, methyl ester, (Z)-(28.02%), cis-13-eicosenoic acid, methyl ester (15.35%), 9,12- octadecadienoic acid -(Z)-, methyl ester (12.75%) and hexadecanoic acid, methyl ester (8.63%). The antioxidant activity ofRaphanus sativeusessential oilwas conducted by different methods: metal chelating, free radical (DPPH∙) and superoxide anion scavenging activity compared to standard antioxidants: BHT (butylated hydroxyl toluene), BHA (butylated hydroxyl anisole) and trolox. In the DPPH assay, the studied oil showed IC50: 68.65±1.23 close to that of the standard antioxidant: butylated hydroxyl toluene (BHT)–(IC50 61.11±1.78). In the superoxide anion scavenging assay, the oil sample gave: IC50 123.19±7.63 while the positive controls showed: BHT(IC50, 60.5±0.22) and Trolox (IC50 54.98±0.17). However, in the metal chelating assay the oil showed IC50:132.53±0.80) while the positive control -EDTA gave: IC50 7.05±0.29.
Keywords Raphanus sativus L., Oil, GC–MS Analysis, Antioxidant Activity.