Isolation and Characterisation of β-Amyrin from Stem Bark of Dacryodes edulis

Abstract D. edulis is widely applied in Nigeria for treatment body pains, cough, diarrhea and fever. The study aims at isolating and characterizing compounds from the ethyl acetate and methanol extract of the plant stem bark. Healthy stem bark of the plant was collected, washed with distilled water, air dried, soxhlet extracted sequentially using ethyl acetate and methanol and concentrated to obtain the ethyl acetate and methanol extracts respectively. Column and thin layer chromatography were used for isolation and purification of the respective solvent extracts. 1HNMRand 13CNMR were employed for structural elucidation of the isolates. The result from the methanolic extract revealed a white crystal solids PDES-16 whose 1HNMR and 13CNMR spectra data agrees reasonably with literature for the compound β-amyrin. From the result, this is the first report of the isolation of β-amyrin from the methanolic extract of D. edulis.

Keywords Unbranded Soymilk, Bacteria, Public Health, Good Quality, Beverage, Coliforms, Antibiotics.

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Updated: January 20, 2024 — 7:47 am