Abstract The compound 3-amino- 4-imino 6, 9 dimethyl- pyrazolo-[3, 4-e] pyrimido [2, 3-b] [1, 3] benzothiazole(IV) was obtained by the reaction of 3-Cyano -6, 9-dimethyl -2-methylthio -4-imino -4H-pyrimido [2, 1-b] [1, 3] benzothiazole (III) with Hydrazine hydrate (80%) in presence of anhydrous K2CO3 in DMF as solvent, and screened their in-vitro anticancer activity by the use of Developmental therapeutic program (DTP) as a computational technique. DTP selection process helps to design and study the anticancer agent, this research facility developed by National Cancer Institute, Maryland, USA. Selections of samples were carried by DTP process, screened their in-vitro anticancer activity against 60 human cancer cell lines, compounds exhibited better anticancer activity.
Keywords Pyrazolo Pyrimido Benzothiazoles, Anticancer activity, NCI Maryland USA.