Abstract In this study the active crude methanol seed extract of pricralimanitida was partition and evaluated for larvicidal activity against Aedes aegypti. About 50g of the active crude methanol extract was dissolve in sufficient quantity of water. This was extracted with sufficient quantity of n-hexane exhaustively; after this it was also exhaustively extracted with sufficient quantity of ethyl acetate. The n-hexane, ethyl acetate and the aqueous portions were dried and the extracts evaluated for larvicidal activities. The evaluation of the larvicidal activities on Aedes aegypti larva was carried in accordance to the method of WHO, 2005 guideline. The result showed that after the partition extraction, the yield was 8.5, 17.2 and 74.3% for n-hexane, ethyl acetate and the aqueous portions respectively. The result of the evaluation the larvicidal activities showed that the n-hexane portion gave the highest activity with LC50 of 0.03, 0.013 and 0.0048mg/ml after 24, 48 and 72 hours respectively. Ethyl acetate portion has LC50 of 0.98, 0.53 and 0.26mg/ml after 24, 48 and 72 hours respectively. While the aqueous part has LC50 of, 10.05, 4.35 and 3.42mg/ml after 24, 48 and 72 hours respectively. From the result the n-hexane and the ethyl acetate portion are recommended for further evaluation and isolation of the active constituent(s).
Keywords seed extract, larvicidal, n-hexane, ethyl acetate, partition.