Abstract According to the World Health Organization, 80% of the African population use herbal remedies for their primary health care and have encouraged the production of improved traditional medicines. Since traditional medicine consists often ofa combination of plants or parts of plants, our work focused on the evaluation of the antibacterial, antioxidant and cytotoxic activity of the combination of leaves and bark of Dacryodes edulis. The leaves and bark of D. edulis were harvested, cut up, dried and crushed separately. The extraction was carried out by macerating the various powders in ethanol / water solvent (70/30).The extracts obtained were then subjected to phytochemical analysis by colorimetric reactions followed by the evaluation of the antibacterial potential of the combination of our extracts by the Checkerboard method. The antioxidant properties of the extracts were evaluated by the DPPH radical scavenging method and Ferric ion reduction and the cytotoxicity test on the Vero cell line by the colorimetric method with resazurin. The bark and leaf extraction yields were 8.1 and 9%, respectively. The phytochemical study revealed the presence of alkaloids, polyphenols, flavonoids, tannins, saponosides and terpenoids. All our extracts were non-cytotoxic with CC50rangingfrom 88.685 to 110.85 ± 5 µg / ml. The combined synergistic effect (FICI) of extracts against Klebsiella pneumoniae ATCC 700603 was 0.16. The extracts had DPPH scavenging IC50s ranging from 48 to >500 ± 3 µg / mL. The reducing power (OD) of the extracts on ferric ions ranged from 2.20 to 2.25 at a concentration of 125 µg / ml. The activities of D. edulis extracts suggest that the latter would be a potential source of active principle.
Keywords Dacryodes edulis, antibacterial combination, antioxidant, cytotoxicity.