Abstract This work was carried out at Jigawa Research Institute Biotechnology Laboratory Kazaure to determine callus induction potentials of 30 wheat genotypes using matured embryo as ex-plant source under different 2,4-Dichlorophenoxyacetic Acid (2,4-D) levels. The experiment had six treatments (control, 1mgl1 2,4-D, 2mgl1 2,4-D, 3mgl1 2,4-D, 4mgl1 2,4-D, and 5mgl1 2,4-D,) including control with each replicated three times and the percentage taken with callus induction frequency which ranged from 32% to 80%. Meanwhile maximum callus among the genotypes were noticed in varieties 8 followed by varieties 27, 28 and 7 with respective callus induction percentages of 79.99%, 79.99% and 77.33%, with minimum callus frequency of 32% was recorded by varieties 19, while 13 had callus induction potential of 36%. The best callus production was recorded at the treatment levels of 5mgl-1 (78.878%), callus recorded at 5mgl-1 were more granular and morphogenic in nature. However higher mean values for days to callus were at varieties 5 (6.60), 4 (6.40), 21 (6.40) and 26 (6.40), with the least mean values for days to callus at varieties 8 (5.40) and 7 (5.60), while the least mean values for days to callus was recorded at 5mgl-1 (4.69) and levels of 2, 4-D, while the higher mean values for days to callus was scored at 2mgl-1 (7.06) levels of 2, 4-D. The weight ranged from 62.24mg mean value to 85.14mg mean values. Genotype 8 had the maximum mean weight across treatments (85.14mg) followed by genotypes 7 (82.5mg), 28 (81.56mg) and 2 (81.46mg) respectively while the minimum weight values were noticed in genotypes 3 (62.24mg), 26 (64.48mg) and (64.9mg). However the maximum weight of callus according to treatments was observed at the level of 5mgl-1 of 2, 4-D with mean value 105.7mg f. while the minimum mean weight values were recorded at 1mgl-1 (31.1mg) 2, 4-D.
Keywords Wheat; Callus; Mature seeds; 2,4-dichlorophenoxyacetic acid.