Abstract The liver is the most vulnerable organ against the side effects of xenobiotics. Aminoglycosides especially gentamicin are the widely used antibiotics against Gram negative bacteria. Cefotaxime is a broad spectrum cephalosporin antibiotic used mainly against Gram positive bacteria. Metronidazole is a chemotherapy used in treatment of protozoal and anaerobic bacterial infections. Combination therapy has complementary and synergistic mechanisms of action. The Present investigation focused on evaluating the effect of curcumin on hepatic alterations of gentamicin, cefotaxime, metronidazole, and their combinations. For this study, eighty eight male rats were classified into eleven groups. (First): Served as control, (Second): Curcumin (200mg/kg.b.wt.,p.o.), (Third): Gentamicin (80 mg/kg.b.wt., i.m), (Fourth): Cefotaxime (540 mg/kg.b.wt., i.m), (Fifth): Metronidazole (135 mg/ kg.b.wt., p.o.), (Sixth): Gentamicin with cefotaxime, (Seventh): Gentamicin with cefotaxime and metronidazole, (Eighth): Curcumin one hour prior gentamicin, (Ninth): Curcumin one hour prior cefotaxime, (Tenth): Curcumin one hour prior gentamicin and cefotaxime, (Eleventh): Curcumin one hour before gentamicin, cefotaxime and metronidazole for 14 successive days. Serum was prepared for measuring of Alkaline phosphate (ALP), Alanine aminotransferase (ALT) and Aspartate aminotransferase (AST), liver was removed for homogenate measurements, histopathological & immunohisto-chemical examination. Administration of curcumin indicated a significant (p < 0.05) decrease of ALP, ALT, AST, malondialdehyde (MDA) and tumor necrosis factor–alpha (TNF-α) and elevation in glutathione (GSH), catalase (Cat), superoxide dismutase (SOD), and glutathione peroxidase (GPx) of liver homogenate. Our data showed that curcumin could protect the liver alterations by blocking oxidative damages.
Keyword: Gentamicin, Cefotaxime, Metronidazole, Curcumin, Liver.