Evaluation of in-vitro antioxidant, anti-inflammatory and diuretic potential of an ethanol extract of Sanseviera liberica leaves on wistar albino rats

Abstract

Sansevieria liberica is a genus of floweringplant in the family of Asparagaceae, it is a medicinal plant mostly used in the herbal medicine to cure Abdominal pains, diarrhea, piles, asthma and snakebites. Cold ethanol extract in leaves of Sansevieria liberica were investigated on Anti-inflammatory and Diuretic activities in albino rats that were compared with the standard drugs Aspirin (300 mg/kg) and furosemide (40 mg/kg) respectively. The extract were administered based on high and low doses, 400 mg/ml, 200 mg/ml, 100 mg/ml, 50 mg and 25 mg/ml, the extracts of  Sansevieria liberica leaves produced a doses-dependent inhibition of acid-induced rat paw oedema, a parameter to show that it has anti-inflammatory activity. The extracts at high concentration were found to induce inflammation with time interval. Highest inhibition was seen in extract with high doses. In diuretics, it shows a low reduction at low concentration of 50 mg and 25 mg respectively. The results obtained were subjected to one-way ANOVA at p < 0.05) level of significance. When compared to the negative control (the normal saline) and the positive control, (the furosemide), the volume of urine level decreased significantly (p<0.05) in rats that received 50 mg and 25 mg/ml of the extract. The urine volume levels was insignificant (p>0.05) in rats given 400 mg, 200 mg and 100 mg/ml of the extract. The antioxidant activity was determined using nitric oxide, 2,2–diphenyl-1-picrylhydrazyl, lipid peroxidation free radical scavenging activities. The plant extract showed (95.75 %) inhibition in nitric oxide at the highest concentration (200 µg/ml) used. This is higher when compared to lipid peroxidation (95.22 %) and 2,2-diphenyl-1- picrylhydrazyl (92.48 %) respectively. The percentage inhibition of the plant extract showed low inhibition concentration as against 50 % (IC50). Isolation and purification of the specific bioactive principles could serve to improve the free radical scavenging potency of the plant.  Conclusively, this study confirms that Sansevieria liberica has anti-inflammatory, antioxidant and diuretics efficacy.

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Updated: May 2, 2018 — 5:13 am
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