Abstract The effect of arbuscular mycorrhizal (AM) fungi on root colonization, growth and nutrient content of (Coleus aromaticus) was investigated as complete randomized design with 4 treatments and 4 replications. Fungi inoculation treatments consisted: Gf (Glomus fasciculatum), Glomus margarita and NM (non-mycorrhizal). The results showed mycorrhizal plants significantly had higher shoot and root dry weight, as well as N, P, K, Ca, Fe, Cu and Mg concentration compared to non-inoculated plants. The effect of AM fungi inoculation on the root colonization, growth parameters dry weight of Coleus aromaticus are more pronounced with G. fasciculatum than other AM fungi. It is concluded that G. fasciculatum was more effective than other species, which may indicate effective symbiotic potential of this strain with Coleus roots.