Abstract This study was aimed at investigating the effect of Trévo dietary supplement (TDS) in caffeine induced oxidative stress in albino rat models. Thirty healthy male albino rats of 12 weeks old were divided into five groups with six rats in each group using a completely randomized design (CRD). The rats were treated with combinations of caffeine and TDS orally via oral gavage. The treatment lasted for 65 days. Results of the oxidative stress markers showed a statistically significant (P<0.05) decrease in the plasma level of superoxide dismutase (SOD), catalase activity (CAT) and glutathione peroxidase (GPx) in caffeine treated groups when compared with the control group; also with an increase in the level of malondialdehyde (MDA) in caffeine treated rats. However, TDS attenuated the caffeine-induced oxidative stress in the albino rat models in a dose dependent manner. This study therefore, reveals that TDS is effective in attenuating caffeine-induced oxidative stress in albino rat models.