Abstract Three precise, accurate and sensitive methods were developed for the determination of Trospiumchloride in presence of its degradation product (acid and alkaline). Method (A) was atomic absorption spectroscopic (AAS), it is based on the formation of ion-pair precipitate complex (1:1) with ammonium reineckate. The precipitate and the filtrate were used to determine the concentration of the drug by direct and indirect way respectively at 357.9 nm. Method (B) was based on the formation of ion-pair association complex (1:1) with bromocresol purple (BCP), bromophenol blue (BPB) and thymol blue (TB). The coloured products were measured at 412.0±2.0 nm. Method (C) was based on the conductometric determination of Trospium chloride by titration with ammonium reneickateat 20°C and the conductance of the solution is measured as a function of the volume of titrant. Linearity ranges for method (A) were 8.0–120.0 μg/mL and 10.0–120.0 μg/mL for the direct and indirect procedures, respectively. In case of method (B) the linearity ranges were 3.0–20.0 µg/mL for BCP, 2.0–25.0 µg/mL for BPB and 5.0–35.0 µg/mL for TB, while the linearity range was 100.0–1000.0 μg/mL for method (C). Statistical comparison between the results obtained by these methods and the official method.
Keywords Trospium chloride, Stability Studies, Atomis Absorption Spectroscopy, Conductometric, Ion-pair complex, Ammonium reineckate, TB, BPB, BCP, Benzilic acid