Cyclic Voltametry study of Interaction of Calf Thymus Deoxyribonucleic Acid (ct-DNA) with an Anticancer Analogue Drug 10-Molybdo 2-Vanado Phosphoric Acid

Abstract In this paper, the electrochemical behavior of [H5PMo10V2O40] modified electrode glassy carbon electrode ([H5PMo10V2O40]/GCE) and its interaction with calf thymus DNA (ctDNA) were investigated using voltammetry.

Modified electrode showed two pair of peaks at A1 (0.00) and A2 (0.179) during the anodic peaks sweep, while the counterpart to the two cathodic peaks are C1 (-0.014) and C2 (0.142) respectively in cyclic voltammetry. The redox property of this modified electrode at various solution pH values and various scan rates were studied using cyclic voltammetry. The linear plot of peak potential EP versus pH was obtained with corresponding slopes 52 m V/ pH. The apparent charge transfer rate constant, ks, and transfer coefficient, α, for electron transfer between [H5PMo10V2O40] and GCE were calculated as 25.203 ± 3.586 and 0.514 (in pH=3), respectively. Binding constant (K = 4.65 X 104 M-1) and binding site size (s =0.5) of modified electrode by ctDNA were obtained by nonlinear fit analysis of voltammetric data based on an electrochemical equation, which was derived to examine the interaction of DNA and reversibly electroactive compounds.

[Full Text: PDF]

Updated: May 7, 2017 — 3:29 pm