Abstract Oxidative stress is associated with increased production of oxidizing species (from reactive oxygen species or transition metals) or a significant decrease in the effectiveness of antioxidant defense. Aluminum toxicity was investigated using antioxidant status in serum of male Wistar albino rats. Antioxidants studied in this work include superoxide dismutase (SOD) and glutathione S- transferase (GST). The results show that specific activities of SOD and GST significantly increased (P<0.05) and decreased (P<0.05) in the test animals given 0.38, 3.8, and 38 mg/kg body weight as compared to the control after days 7 and 14 respectively. The results suggest that aluminum intoxication mobilizes antioxidants indicating possible oxidative stress.