Abstract The present study was carried to evaluate antibacterial activity of water extracts of honey against some selected bacteria (Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae and pseudomonas aeruginosa) using microdilution method (MIC) at different concentrations (100-0.195mg/ml) and well diffusion method (200 mg/ml). The inhibitory potency of the honey from different sources varied on the test organisms and all the test organisms were susceptible to the honey extracts at different concentration. The water extracts of the sample 2 showed powerful antimicrobial activity against Gram-positive and Gram-negative bacteria and sample 1 showed acceptable degree in control of growth Gram-negative other than Gram-positive bacteria. The water extract from sample 2 showed powerful antimicrobial activity against S. aureus and E. coli with MIC value 6.25 mg/ml and inhibition zoon (38-29mm) respectively. These results were revealed the importance of tested extracts in control of some human pathogenic micro-organisms.