Abstract The use of herbal remedies, including corn silk, classified as Stigma Maydis or Zea mays, are popular as an alternative to standard Western allopathic medicine for a variety of problems, including prostate disorders, a diuretic as well as for bedwetting and obesity. In the present study the antidiabetic potential of methanol extract of cooked, dried and pulverized Stigma Maydis was assessed in alloxan-induced diabetic albino mice. Hyperglycemia was induced by the injection of 150 mg/kg (i.p.) of alloxan monohydrate freshly dissolved in physiological saline. Doses (250, 500 and 750 mg/kg) per os, of the extract were separately administered to three groups of five diabetic mice each in the study. The activity was compared with reference standard glibenclamide (2 mg/kg, p.o.) and negative control of physiological saline. Treatment of the alloxan-induced diabetic mice with the methanol extract of Stigma Maydis decreased the raised blood glucose levels in a dose-dependent manner. The methanol extract exhibited relatively significant (P<0.05) anti-diabetic effect after 72 h and 96 h of treatment with the doses of 500 and 750 mg/kg body weight, respectively. Results of phytochemical screening of the methanol extract of cooked Stigma Maydis have indicated the presence of cardiac glycoside, steroids, terpenoids, alkaloids, flavonoids, carbohydrates and anthraquinones.