Abstract As an alternative medicine to treat diabetes mellitus, many herbal drugs are being studied throughout the world. In the present study, an attempt was made to investigate the anti-diabetic activity of Bambusa Arundinacea. Leaves extracts in different dosages (200, 500 and 600 mg/kg b.w.) in alloxan induced diabetic albino rats. The drug was administered orally for 20 days in different groups of animal, with each group containing 6 animals. The efficacy of this drug was compared with the diabetic rats. The blood glucose level of different groups was studied. Our study clearly showed that significant (P < 0.05) anti-diabetic activity in diabetic animals.