Healing various ailments is probably the most outstanding quality of plants that mankind is dependent upon from ages. Plants are useful for treating a variety of diseases of man and also animals. The Chichawatni Plantation Forest located in Sahiwal District is designated as Reserve Land. This involves huge biological landscape, constituting numerous valuable medicinal plants. The aim of this specific study has been to document conventional knowledge of plant usage and its significance to the local people; identifying the flora; assess the abundance and measures necessary to be taken for protection of plant species. Ethno-medicinal questionnaire had been got filled by people residing in the periphery of Chichawatni Plantation Reserved Forest. This questionnaire provided information about many plants growing at study area. Some of the plants having medicinal value included Trianthema portulacastrum, Calotropis procera, Xanthium strumarium, Cordia myxa, Chenopodium album, Citrullus colocynthis, Cuscuta reflexa, Cyperus rotundus etc. Local people use them for treating anemia, paralysis, fever, jaundice, piles, diabetes, diarrhea, motion respectively. Some of the plants such as Capparis deciduas, Prosopis cineraria, Salvadora oleoides, Suaeda fruticosa and Malvastrum coromendelianum were present in less number perhaps because of overexploitation as having greater medicinal importance, indicating requirement of their preservation. There is need for sustainable use and conservation of useful medicinal plants in the area so that their constant availability is ensured.